However, they may be sterilised using the gas ethylene oxide formaldehyde. Glass is considered safe for autoclaving however, borosilicate glassware is recommended because it is composed of silica and boron trioxide which are known to withstand high pressures better than other glass materials. In terms of closure liners, rubber and Teflon are known to be safe for autoclaving as well.
Make sure that you clean and rinse all objects with distilled water prior to autoclaving to avoid any reactions with pre-used chemical solutions. Another precautionary measure to take it to make sure that bottles are loosely capped in order to prevent explosions from high pressures.
For bottles that are not made of safety glass or are basically not Pyrex then cover them with aluminum foil. Other objects that can be autoclaved include cotton plugs, labware, gauze, plastic caps, filters, water, pipettes, and stainless steel, nutrient media. However, metal instruments or objects are not recommended for autoclaving because this can lead to the formation of rust.
When autoclaving glass and plastic, it is important that they are cleaned properly and do not contain hazardous materials that can create toxic vapors and explosives.
Listed below is a guide of incompatible autoclaving materials :. Autoclaves are trusted sterilisation devices based on their capabilities to efficiently and effectively kill any traces of harmful microbial life.
However, before autoclaving anything, make sure that you are aware of what can and cannot be autoclaved. Glassware is generally safe for autoclaving, but make sure it does not contain any hazardous materials and that it is not household glassware, as this can lead to dangerous accidents and other harmful consequences. On the other hand, plastic has a few more stringent restrictions. Increased cycle time may be necessary depending upon the make-up and volume of the load.
The rate of exhaust will depend upon the nature of the load. Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids. Plastic Polypropylene is an inexpensive resin that can resist autoclave temperatures.
Polypropylene containers are often used as secondary containers to hold materials that are autoclaved. Polycarbonate can also withstand high temperatures. Gloves Gloves must be placed inside of an autoclavable biohazard bag and exposed to a steam setting; gloves will melt slightly but will not burn when autoclaved in this manner.
Stainless steel Most metals are designed for extreme conditions and are intended to be sterilized. Make sure to remove any plastics, liners and other items that may melt or combust. Paper Paper is combustible and should not be placed directly inside an autoclave. It should be autoclaved in a waste bag on a biobag setting to prevent fire. Media Solution No liquid should be sealed in a container and autoclaved. They should autoclaved in a steam producing cycle. Pipette tips Most pipette tips are autoclavable.
Some of these tips are plastic, some are high density polyethylene. In general, pipette tips should only enter the autoclave as waste inside of an approved biohazards bag and always sterilized on a steam-producing setting. Before using the autoclave, check inside for any items left by the previous user that could pose a hazard.
Clean the drain strainer before loading the autoclave. Always place items in a secondary container. Do not overload or package bags too tightly. Leave sufficient room for steam circulation. If necessary, place container on its side to maximize steam penetration and avoid entrapment of air. If autoclaved repeatedly, PSF will eventually fail under high-stress applications. After repeated autoclaving, PC shows some loss of mechanical strength and may not function well under high-stress applications such as centrifugation.
Note: Some transparent plastics may absorb minute amounts of water vapor and appear cloudy after autoclaving. The clouding will disappear as the plastic dries. Like this: Like Loading Leave a comment Cancel reply Your email address will not be published.
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